Ikhithi yokutholwa kwe-Mucorales Molecular (PCR yesikhathi sangempela)
Isingeniso Somkhiqizo
I-FungiXpert® Mucorales Molecular Detection Kit (Real-time PCR) isetshenziswa ekutholweni kwekhwalithi ye-Mucorales DNA ku-BALF, isikhwehlela namasampula e-serum.Ingasetshenziswa ukuxilonga okuyisizayo kweziguli ezigula kakhulu ezisolwa nge-Mucor mycosis kanye neziguli ezilaliswe esibhedlela ezinokuzivikela okuphansi.
Njengamanje, izindlela ezivame ukusetshenziswa zokuthola imitholampilo ye-Mucorales isiko kanye nokuhlolwa kwe-microscopic.I-Mucorales ikhona emhlabathini, indle, utshani kanye nomoya.Ikhula kahle ngaphansi kwezimo zokushisa okuphezulu, umswakama ophezulu kanye nomoya ompofu.I-Mucor mycosis uhlobo lwesifo esinemibandela esibangelwa yi-Mucorales.Iziguli eziningi zingenwa yilesi sifo ngokuhogela izinhlamvu ezisemoyeni.Amaphaphu, ukona kanye nesikhumba yizindawo ezivame kakhulu ukutheleleka.Ukubikezelwa kokutheleleka okujulile kwe-Mucorales kubi futhi ukufa kuphezulu.Isifo sikashukela, ikakhulukazi i-ketoacidosis yesifo sikashukela, ukwelashwa kwe-glucocorticoid, izifo ze-hematological, amangqamuzana e-hematopoietic stem kanye neziguli zokufakelwa izitho eziqinile.
Izici
| Igama | Ikhithi yokutholwa kwe-Mucorales Molecular (PCR yesikhathi sangempela) |
| Indlela | I-PCR yesikhathi sangempela |
| Uhlobo lwesampula | Isikhwehlela, uketshezi lwe-BAL, iSerum |
| Ukucaciswa | 20 test/kit, 50 test/kit |
| Isikhathi sokutholwa | 2 h |
| Izinto zokutholwa | Mucorales spp. |
| Ukuzinza | Izinzile izinyanga eziyi-12 ku -20°C |
| Ukuzwela | 100% |
| Ukucaciswa | 99% |
Mayelana ne-Mucormycosis
I-Mucormycosis isifo sesikhunta esiyingozi kodwa esingajwayelekile esibangelwa iqembu lezibungu ezibizwa ngokuthi ama-mucormycetes.Lezi zikhunta zihlala kuyo yonke imvelo.I-Mucormycosis ihlasela kakhulu abantu abanezinkinga zempilo noma abaphuza imithi eyehlisa amandla omzimba okulwa namagciwane nokugula.Ngokuvamile kuthinta ukona noma amaphaphu ngemva kokuhogela izinhlamvu zesikhunta emoyeni.Kungenzeka futhi esikhumbeni ngemva kokusikwa, ukusha, noma olunye uhlobo lokulimala kwesikhumba.Isigameko sangempela se-mucormycosis akwaziwa futhi cishe asithathwa kancane ngenxa yobunzima bokuxilongwa kwe-antemortem.
Ukutheleleka okubangelwa ama-Mucorales (okungukuthi, ama-mucormycoses) ahlasela kakhulu, aqala kakhulu, athuthuka ngokushesha, futhi ngokuvamile abulalayo amagciwane e-angioinvasive fungal.Lezi zikhunta kuthiwa zitholakala yonke indawo ngokwemvelo futhi zitholakala kabanzi kuma-substrates e-organic.Cishe ingxenye yawo wonke amacala e-mucormycosis abangelwa i-Rhizopus spp.Izici zobungozi ezihlobene ne-mucormycosis zihlanganisa i-neutropenia ende kanye nokusetshenziswa kwe-corticosteroids, izifo ze-hematological, i-aplastic anemia, i-myelodysplastic syndromes, ukufakelwa kwesitho esiqinile noma i-hematopoietic stem cell, ukutheleleka kwegciwane lomuntu, isifo sikashukela kanye ne-metabolic acidosis, ukugcwala kwensimbi, ukusetshenziswa kwe-deferoxamine, ukusha, ukungondleki kahle, ukuguga ngokweqile nokusebenzisa kabi izidakamizwa.
Inzuzo
- Kuyavumelana nezimo
Uhlobo lwesampula luyinketho phakathi kwesikhwehlela ne-BALF - Inembile
I-1.I-reagent igcinwa ku-PCR tube ngendlela ye-powder efriziwe ukuze kuncishiswe amathuba okungcola.
2.Lawula ngokuqinile ikhwalithi yokuhlola
3.Imiphumela yokuqapha enamandla ikhombisa izinga lokutheleleka
4.Ukuzwela okuphezulu nokucacisa
Oda Ulwazi
| Imodeli | Incazelo | Ikhodi yomkhiqizo |
| I-FMPCR-20 | Izivivinyo ezingama-20/ikhithi | I-FMPCR-20 |
| I-FMPCR-50 | Izivivinyo ezingama-50/ikhithi | I-FMPCR-50 |
