Ukutholwa kwe-K-Set kwe-IMP engamelana ne-Carbapenem (I-Lateral Flow Assay)
Isingeniso Somkhiqizo
I-Carbapenem-resistant IMP Detection K-Set (Lateral Flow Assay) iwuhlelo lokuhlola lwe-immunochromatographic oluhloselwe ukutholwa kwekhwalithi ye-IMP-type carbapenemase kumakoloni ebhaktheriya.I-assay iwukuhlolwa kwelabhorethri yokusebenzisa incwadi kadokotela engasiza ekuxilongweni kohlobo lwe-IMP-type carbapenem resistant.
Izici
| Igama | Ukutholwa kwe-K-Set kwe-IMP engamelana ne-Carbapenem (I-Lateral Flow Assay) |
| Indlela | I-Lateral Flow Assay |
| Uhlobo lwesampula | Amakoloni amagciwane |
| Ukucaciswa | Izivivinyo ezingama-25/ikhithi |
| Isikhathi sokutholwa | 10-15 imiz |
| Izinto zokutholwa | I-Enterobacteriaceae engamelana ne-Carbapenem (CRE) |
| Uhlobo lokutholwa | I-IMP |
| Ukuzinza | I-K-Set izinzile iminyaka emi-2 ku-2°C-30°C |
Inzuzo
- Ngokushesha
Thola umphumela phakathi kwemizuzu engu-15, izinsuku ezi-3 ngaphambi kwezindlela zokuthola ezivamile - Kulula
Kulula ukuyisebenzisa, abasebenzi baselabhorethri abajwayelekile bangasebenza ngaphandle kokuqeqeshwa - Inembile
Ukuzwela okuphezulu nokucacisa
Umkhawulo wokutholwa ophansi: 0.20 ng/mL
Iyakwazi ukuthola iningi lezinhlobo ezincane ezivamile ze-IMP
- Umphumela onembile
Asikho isidingo sokubala, umphumela wokufunda obonwayo - Ezomnotho
Umkhiqizo ungathuthwa futhi ugcinwe ekamelweni lokushisa, ukunciphisa izindleko
Ukubaluleka kokuhlolwa kwe-CRE
Ngokuhlangene, ama-Enterobacterales yiqembu elivame kakhulu lamagciwane abangela izifo ezihambisana nokunakekelwa kwezempilo.Amanye ama-Enterobacterales angakhiqiza i-enzyme ebizwa ngokuthi i-carbapenemase eyenza ama-antibiotic afana ne-carbapenems, ama-penicillin, nama-cephalosporins angasebenzi.Ngenxa yalesi sizathu, i-CRE ibizwe ngokuthi “amabhaktheriya aphuphayo” ngoba mancane ama-antibiotics, uma ekhona, asele ukuze elapha izifo ezibangelwa yila magciwane.
Amagciwane aphuma emndenini wakwa-Enterobacterales, kuhlanganise nezinhlobo ze-Klebsiella kanye ne-Escherichia coli, angakhiqiza i-carbapenemase.Ama-Carbapenemase avame ukukhiqizwa kusuka kuzakhi zofuzo ezitholakala ezintweni ezidlulisekayo ezingasakaza ukumelana kalula kusuka egciwaneni kuye egciwaneni kanye nomuntu kuye komunye.Futhi ngenxa yokusetshenziswa kabi kwemithi elwa namagciwane kanye nezindlela ezilinganiselwe ezithathwayo ukuze kuvinjelwe ukusakazeka, inkinga ye-CRE eyanda ngokuphawulekayo iba usongo lokuphila emhlabeni wonke.
Imvamisa, ukusabalala kwe-CRE kungalawulwa yilokhu:
- Ukuqapha izifo ze-CRE
- Hlukanisa iziguli ezine-CRE
- Ukukhipha imishini yezokwelapha ehlaselayo ngaphakathi emzimbeni
- Qaphela lapho ubeka ama-antibiotics (ikakhulukazi i-carbapenems)
- Ukusebenzisa amasu ahlanzekile ahlanzekile ukuze kuncishiswe ukusabalala kokutheleleka
- Landela ngokuqinile inkambiso yokuhlanza ilebhu
……
Ukutholwa kwe-CRE kunenani elikhulu ekulawuleni ukusabalala.Ngokuhlola kusenesikhathi, abahlinzeki bezempilo banganikeza ukwelashwa okunengqondo ezigulini ezisengozini ye-CRE, futhi bathole ukuphathwa kokulaliswa esibhedlela.
Uhlobo lwe-IMP carbapenemase
I-Carbapenemase ibhekisela ohlotsheni lwe-β-lactamase olungase okungenani i-hydrolyze imipenem noma i-meropenem ngokuphawulekayo, kuhlanganise no-A, B, D izinhlobo ezintathu zama-enzyme ahlukaniswa ngesakhiwo se-Ambler samangqamuzana.Phakathi kwazo, i-Class B yi-metallo-β-lactamases (MBLs), kuhlanganise nama-carbapenemase afana ne-IMP, i-VIM ne-NDM,.I-IMP-type carbapenemase, eyaziwa nangokuthi imipenemase metallo-beta-lactamase ekhiqiza i-CRE, iwuhlobo oluvame kakhulu lwama-MBL atholakele futhi lusuka ku-subclass 3A.Ingakwazi ukwenza i-hydrolyze cishe wonke ama-antibiotic e-β-lactam.
Ukusebenza
- Engeza amaconsi angu-5 esixazululo sokwelashwa okuyisampula
- Dip amakholoni ebhaktheriya nge-loop yokugoma elahlwayo
- Faka iluphu kushubhu
- Engeza u-50 μL emthonjeni we-S, linda imizuzu engu-10-15
- Funda umphumela
Oda Ulwazi
| Imodeli | Incazelo | Ikhodi yomkhiqizo |
| I-CPI-01 | Izivivinyo ezingama-25/ikhithi | I-CPI-01 |
