Lolu chungechunge lwezindlela luwukuhlola ngokusebenzisa i-antigen ethile yegciwane ukuthola amasosha omzimba ku-serum yeziguli, okuhlanganisa ukutholwa kwamasosha omzimba e-IgM kanye nokukalwa kwamasosha omzimba e-IgG.Amasosha omzimba e-IgM ayanyamalala emasontweni ambalwa, kuyilapho amasosha omzimba e-IgG ephikelela iminyaka eminingi.Ukusungula ukuhlonzwa kokutheleleka ngegciwane kufezwa nge-serologically ngokubonisa ukwenyuka kwe-antibody titer kugciwane noma ngokubonisa amasosha omzimba alwa namagciwane ekilasi le-IgM.Izindlela ezisetshenzisiwe zihlanganisa ukuhlolwa kwe-neutralization (Nt), ukuhlolwa kwe-complement fixation (CF), ukuhlolwa kwe-hemagglutination inhibition (HI), nokuhlolwa kwe-immunofluorescence (IF), i-hemagglutination yokwenziwa, kanye ne-immunodiffusion.
A. Izivivinyo zokungathathi hlangothi
Ngesikhathi sokutheleleka noma isiko lamaseli, igciwane lingavinjelwa i-antibody yalo ethile futhi lilahlekelwe ukusuleleka, lolu hlobo lwe-antibody luchazwa njenge-neutralization antibody.Ukuhlolwa kokungathathi hlangothi ukuthola i-antibody eyenza i-neutralization ku-serum yeziguli.
B. Gcwalisa Izivivinyo Zokulungisa
Ukuhlolwa kokulungisa okuhambisanayo kungasetshenziselwa ukubheka ubukhona be-antibody ethile noma i-antigen ku-serum yesiguli.Ukuhlola kusebenzisa amaseli egazi abomvu ezimvu (SRBC), amasosha omzimba alwa ne-SRBC kanye nomphelelisi, kanye ne-antigen ethile (uma ibheka i-antibody ku-serum) noma i-antibody ethile (uma ibheka i-antigen ku-serum).
C. I-Hemagglutination Inhibition Assays
Uma ukuqoqwa kwegciwane kusampula kuphezulu, lapho isampula lixutshwa nama-RBC, kuzokwakhiwa i-lattice yamagciwane nama-RBC.Lesi simo sibizwa ngokuthi i-hemagglutination.Uma amasosha omzimba aphikisana nama-hemagglutinins ekhona, i-hemagglutination izovinjelwa.Ngesikhathi sokuhlolwa kwe-hemagglutination inhibition, ukuhlanjululwa okulandelanayo kwe-serum kuhlanganiswa nenani elaziwayo legciwane.Ngemva kokufukamela, ama-RBC ayengezwa, futhi ingxube iyekwa ukuthi ihlale amahora ambalwa.Uma i-hemagglutination ivinjiwe, i-pellet yama-RBCs yakheka ngaphansi kweshubhu.Uma i-hemagglutination ingavinjelwa, ifilimu encane iyakhiwa.
Isikhathi sokuthumela: Oct-15-2020