Kusukela ngoJanuwari 2020 kuya ku-Okthoba 2020, ucwaningo lwendlela yokusebenza lwenziwa esibhedlela sasePisa University olwashicilelwa nge-BMC Microbiology.I-Goldstream®Isikhunta (1-3)-β-D-Glucan Test sasetshenziselwa ukuthola izinga le-BDG kumasampuli e-BAL.Umphumela ubalwe ngu-aI-Automatic Kinetic Tube Reader IGL-200 ngokugcwelekusuka ku-Era Biology.Ucwaningo lubonisa ukuthi i-BDG kufanele icatshangelwe ngevelu yayo yokubikezela engalungile ephezulu.Futhi kube nomphumela owusizo ekukhipheni ukuxilongwa kwe-PCP kuzo zonke iziguli zokulawula okungalungile.
Ingemuva:
Izinga legolide lokuxilonga lamanje leI-Pneumocystis jiroveciiimelelwa ukubona ngeso lengqondo ukhunta kumasampula okuphefumula omtholampilo, njengoketshezi lwe-bronchoalveolar-lavage, okuchaza “okuqinisekisiwe”P. jiroveciiinyumoniya, kuyilapho i-qPCR ivumela ukuchaza ukuxilongwa “okungenzeka”, njengoba ingakwazi ukubandlulula ukutheleleka kusukela ekoloni.Nokho, izindlela zamangqamuzana, njenge-end-point PCR kanye ne-qPCR, ziyashesha, zisebenza kalula futhi zitolika, ngaleyo ndlela zivumela ilabhorethri ukuthi ibuyisele odokotela idatha ewusizo ye-microbiological ngesikhathi esifushane.Ucwaningo lwamanje luhlose ukuqhathanisa i-microscopy nezivivinyo zamangqamuzana kanye nokusebenza kokuxilonga kwe-beta-D-glucan kuketshezi lwe-bronchoalveolar-lavage oluvela ezigulini ezisolwayo.I-Pneumocystis jiroveciiinyumoniya.Uketshezi lwe-bronchoalveolar-lavage oluvela ezifundweni eziyishumi nesishiyagalombili ezinobungozi obuphezulu kanye nezine zokulawula okungekuhle bathole i-methenamine yesiliva ye-Grocott-Gomori, i-end-point PCR, i-RT-PCR, ne-beta-D-glucan assay.
Imiphumela:
Onke amasampula e-microscopically positive bronchoalveolar-lavage (50%) nawo abe nomphumela omuhle nge-PCR yephoyinti lokugcina kanye nesikhathi sangempela nakho konke, kodwa okubili, kube nokuhle futhi ngokulinganisa kwe-beta-D-glucan.I-PCR yephoyinti lokugcina kanye ne-RT-PCR ithole i-10 (55%) kanye ne-11 (61%) kumasampuli angu-18, ngokulandelana, ngaleyo ndlela ikhombisa ukuzwela okuthuthukisiwe uma kuqhathaniswa ne-microscope.Yonke i-RT-PCR ene-Ct<27 iqinisekiswe nge-microscopically, kanti amasampula ane-Ct≥ 27 awazange aqinisekiswe.
Iziphetho:
Umsebenzi wethu ugqamisa isidingo sokubunjwa kabusha kanye nokuchaza kabusha indima yokuxilongwa kwamangqamuzana esimweni somtholampilo esingavamile, njengeP. jiroveciiukutheleleka, okuyisimo somtholampilo esingavamile kodwa esinzima futhi esithuthukayo ngokushesha esithinta ababungazi abangenawo amandla omzimba angazuza kakhulu ekuxilongweni okusheshayo.Iziguli ezikhethwe ngokuqinile, ngokwemibandela yokufaka, umphumela ongemuhle ngezindlela zamangqamuzana ungakhishwaP. jiroveciiinyumoniya.
UFranconi I, uLeonildi A, u-Erra G, et al.Ukuqhathaniswa kwezinqubo ezahlukene ze-microbiological zokuxilongwa kwe-Pneumocystis jirovecii pneumonia ku-bronchoalveolar-lavage fluid.I-BMC Microbiol.2022;22(1):143.Kushicilelwe 2022 May 21. doi:10.1186/s12866-022-02559-1
Isikhathi sokuthumela: Jun-14-2022